![]() For height profiles and elasticity maps of each value and condition, refer to Figure 2-figure supplements 3– 6. Source data please refer to Figure 2-source data 1. The results were presented as mean ± SEM, from 7 to 12 cells per condition as shown in the plots (LFA-1 vs full anybody-set) from six independent experiments for ( A–F) (six donors), from 10 cells (four independent experiments/four donors) for PDMS condition (2.5 MPa) in ( G–J), or from 5 cells (two independent experiments/two donors) for PDMS condition (400 kPa). The Mann-Whitney test ( C), ( D), ( H), and ( I), the Wilcoxon matched-pairs signed rank test ( E), ( F), and ( J) or Mann-Whitney-U-test ( L) was used for analyzing statistical significance. ( H–J, L) Young’s modulus of primary T cells on full antibody (αLFA-1+αCD3+αCD28)-functionalized glass (n = 11, the same dataset as in (D) full antibody), PDMS (2.5 MPa, n = 10), or PDMS (400 kPa, n = 5) substrates. ![]() Either glass coverslips ( A–F) or PDMS ( G–K) were applied as the functionalized surfaces. Height profiles and corresponding elasticity maps, Young’s moduli, and the respective fold changes of human primary CD4 + T cells.
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